1·
BIND
Heavy Chain recognizes receptors on the presynaptic nerve terminal.
RECOMBINANT LIGHT-CHAIN PLATFORM
BEYOND THE HEAVY CHAIN
A New Recombinant Light-Chain Platform for Skin-Focused Neuromodulation
A skin-focused platform exploring recombinant light-chain architecture for a more precise and focused approach to neuromodulation.
LIGHT-CHAIN
PLATFORM
SKIN
FOCUS
O-BLOXIN® / O-BOTLIX®
LEADS
01THE CLASSIC ARCHITECTURE
THE CLASSIC ARCHITECTURE
One neurotoxin. Two functional chains.
Conventional botulinum toxin type A is a 150 kDa core protein composed of two polypeptide chains—a Heavy Chain and a Light Chain—linked by a disulfide bond.
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HEAVY CHAIN
Mediates receptor recognition, membrane translocation and intracellular delivery.
S—S
LIGHT CHAIN
The catalytic component that cleaves SNAP-25.
MECHANISM IN FOUR STEPS
The Heavy Chain delivers. The Light Chain acts.
02·CONVENTIONAL BOTULINUM TOXIN
2·
ENTER
The complex is internalized through receptor-mediated endocytosis.
3·
RELEASE
Light Chain is translocated into the cytosol.
4·
CLEAVE
Light Chain cleaves SNAP-25, reducing acetylcholine release.
Functional endpoint:
THE CLINICAL NEED CHANGED.THE ARCHITECTURE HAD NOT.
Conventional toxin was built for efficient neuronal delivery — not specifically for controlled skin-level formulation.
Sensitive anatomy
The upper face concentrates high-density functional structures.
Multifactorial spread
Toxin diffusion is influenced by muscle contraction, skin dynamics and microanatomy.
New delivery mission
Control at the skin level — not just deep neuromuscular blockade.
Innovation question: a different clinical mission?
WHY EXPRESSION MUSCLESBECAME THE PRIMARY TARGET
Reduce the dynamic force acting on an ageing dermis.
Conventional intramuscular treatment is highly effective because it addresses the motor component of wrinkle formation.
1
EXPRESSION MUSCLE
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Creates repeated movement and skin folding.
2
REDUCED CONTRACTION
Lowers the intensity and frequency of mechanical folding.
3
VISIBLE OUTCOME
Dynamic lines soften and the dermis experiences less repetitive stress.
muscle-driven problem
02THE SCIENTIFIC PIVOT
WHAT IF WE SEPARATED THE CATALYTIC FUNCTION
FROM THE CONVENTIONAL DELIVERY ROUTE?
Keep the active principle. Redesign the route.
CONVENTIONAL ARCHITECTURE
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HEAVY CHAIN
Delivery vehicle
(Structural / Transport)
LIGHT CHAIN
Catalytic function
(Active principle)
fromDELIVERY VEHICLEtoENGINEERED ROUTE
ENGINEERED RECOMBINANT PLATFORM
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LIGHT CHAIN CATALYTIC DOMAIN
Retained activity
(Active principle)
+
ENGINEERED DELIVERY SEQUENCE
Purpose-built route
(Targeted delivery)
“
One question changed everything.
03 · RECOMBINANT PROTEIN ENGINEERING
FROM GENETIC CONSTRUCTTO SELECTED LEAD
A designed plasmid encodes the Light-Chain domain and delivery sequence as one fusion protein.
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Design
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Clone
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Express
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Purify
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Screen
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Select
The host cell expresses the engineered recombinant fusion protein from the beginning.
04TWO LEAD MOLECULES
TWO LEAD MOLECULES EMERGED
One scientific backbone. Two engineered delivery missions.
Two lead molecules. One shared scientific core. Distinct delivery missions.
05 · SCIENCE ACCESS
The right information, at the right access level.
Public scientific storytelling remains separate from professional technical content and NDA-protected evaluation material.
Discover the Science
Light-Chain story, simplified architecture, recombinant platform and approved public visuals.
Explore Professional Science
Qualified professional access for approved mechanism, applications and technical evaluation material.
Protected Evaluation
Confidential composition, unpublished studies, process information and evaluation files after NDA activation.
RAW MATERIAL EVALUATION
Interested in the Light-Chain platform?
Tell us about your company, target market and development objective. The appropriate scientific, technical or NDA pathway can then be opened according to the request.